Date of Award

6-2-2025

Degree Type

Thesis

Degree Name

Master of Environmental Science (M.Env.Sc.)

Department

Agricultural and Environmental Sciences

First Advisor

Christine Ondzighi

Abstract

The inflation of population in the world has led to a high and consistent food demand which has reinvigorated worldwide interest to create high-yielding varieties of legumes. Soybean Glycine max (L.) Merrill, a member of this legume family has become one of the most essential food crops in the United States as well as in the world due its edible oil and protein content up to 20% and 54%, respectively. Because of its high importance as a food crop, major objectives in soybean breeding, improvement of protein and oil production need to be achieved. These objectives can be addressed using biotechnology high-throughput efficient embryogenic cell culture system in soybean capable of (i) Cryopreservation and recovery post-cryopreservation, (ii) Stable genetic transformation and (iii) regeneration from cell to the whole plant, we will ease and fasten genetic modifications of genes of interest and subsequently plant production in Soybean. Thus, three specific objectives are investigated as follows: 1) Establishment, cryopreservation, and recovery of embryogenic cell culture systems. 2) Transformation of established cell culture systems with plasmid vectors specific to crops (pANIC10A and CRISPR.Cas9 technology). 3) Regeneration and characterization of transgenic cell line systems. Promising results showed that the two varieties of soybean used, William 82 and Henderson, produced embryogenic cell systems capable of growth rates and recovery before and after cryopreservation. Interestingly they exhibited high efficiencies (ranging from 68% to 90%) of viability, transformation, and regeneration.

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