Microbial inactivation and cytotoxicity evaluation of UV irradiated coconut water in a novel continuous flow spiral reactor

Authors

Manreet Singh Bhullar, Tennessee State UniversityFollow
Ankit Patras, Tennessee State UniversityFollow
Agnes Kilonzo-Nthenge, Tennessee State UniversityFollow
Bharat Pokharel, Tennessee State UniversityFollow
Sudheer K. Yannam, Tennessee State UniversityFollow
Kanyasiri Rakariyatham, University of Massachusetts Amherst
Che Pan, University of Massachusetts AmherstFollow
Hang Xiao, University of Massachusetts AmherstFollow
Michael Sasges, Aquafine CorporationFollow

Document Type

Article

Publication Date

10-4-2017

Abstract

A continuous-flow UV reactor operating at 254 nm wave-length was used to investigate inactivation of microorganisms including bacteriophage in coconut water, a highly opaque liquid food. UV-C inactivation kinetics of two surrogate viruses (MS2, T1UV) and three bacteria (E. coli ATCC 25922, Salmonella Typhimurium ATCC 13311, Listeria monocytogenes ATCC 19115) in buffer and coconut water were investigated (D₁₀ values ranging from 2.82 to 4.54 mJ·cm^{− 2}). A series of known UV-C doses were delivered to the samples. Inactivation levels of all organisms were linearly proportional to UV-C dose (r² > 0.97). At the highest dose of 30 mJ·cm^{− 2}, the three pathogenic organisms were inactivated by > 5 log₁₀ (p < 0.05). Results clearly demonstrated that UV-C irradiation effectively inactivated bacteriophage and pathogenic microbes in coconut water. The inactivation kinetics of microorganisms were best described by log linear model with a low root mean square error (RMSE) and high coefficient of determination (r² > 0.97). Models for predicting log reduction as a function of UV-C irradiation dose were found to be significant (p < 0.05) with low RMSE and high r². The irradiated coconut water showed no cytotoxic effects on normal human intestinal cells and normal mouse liver cells. Overall, these results indicated that UV-C treatment did not generate cytotoxic compounds in the coconut water. This study clearly demonstrated that high levels of inactivation of pathogens can be achieved in coconut water, and suggested potential method for UV-C treatment of other liquid foods.

Industrial relevance

This research paper provides scientific evidence of the potential benefits of UV-C irradiation in inactivating bacterial and viral surrogates at commercially relevant doses of 0–120 mJ·cm^{− 2}. The irradiated coconut water showed no cytotoxic effects on normal intestinal and healthy mice liver cells. UV-C irradiation is an attractive food preservation technology and offers opportunities for horticultural and food processing industries to meet the growing demand from consumers for healthier and safe food products. This study would provide technical support for commercialization of UV-C treatment of beverages.

Recommended Citation

Manreet Singh Bhullar, Ankit Patras, Agnes Kilanzo-Nthenge, Bharat Pokharel, Sudheer Kumar Yannam, Kanyasiri Rakariyatham, Che Pan, Hang Xiao, Michael Sasges, "Microbial inactivation and cytotoxicity evaluation of UV irradiated coconut water in a novel continuous flow spiral reactor", Food Research International, Volume 103, 2018, Pages 59-67, ISSN 0963-9969, https://doi.org/10.1016/j.foodres.2017.10.004.