Evaluation of a Hydrogel-Based Diagnostic Approach for the Point-of-Care Based Detection of Neisseria gonorrhoeae

Authors

Sumudu R. Perera, University of Saskatchewan
Ali Taheri, Vaccine and Infectious Disease OrganizationFollow
Nurul H. Khan, Vaccine and Infectious Disease Organization
Rajinder P. Parti, Vaccine and Infectious Disease Organization
Stephanie Stefura, National Institute for Nanotechnology
Pauline Skiba, National Institute for Nanotechnology
Jason P. Acker, National Institute for Nanotechnology
Irene Martin, Public Health Agency of Canada
Anthony Kusalik, University of Saskatchewan
Jo-Anne R. Dillon, University of Saskatchewan

Document Type

Article

Publication Date

8-4-2018

Abstract

Eleven primer pairs were developed for the identification of Neisseria gonorrhoeae. The sensitivity and specificity of these primers were evaluated by Real Time (RT)-PCR melt curve analyses with DNA from 145 N. gonorrhoeae isolates and 40 other Neisseria or non-Neisseria species. Three primer pairs were further evaluated in a hydrogel-based RT-PCR detection platform, using DNA extracted from 50 N. gonorrhoeae cultures. We observed 100% sensitivity and specificity in the hydrogel assay, confirming its potential as a point-of-care test (POCT) for N. gonorrhoeae diagnosis.

Recommended Citation

Perera, S.R.; Taheri, A.; Khan, N.H.; Parti, R.P.; Stefura, S.; Skiba, P.; Acker, J.P.; Martin, I.; Kusalik, A.; Dillon, J.-A.R. Evaluation of a Hydrogel-Based Diagnostic Approach for the Point-of-Care Based Detection of Neisseria gonorrhoeae. Antibiotics 2018, 7, 70. https://doi.org/10.3390/antibiotics7030070