Investigating NodGRPs, GOLVEN10 Functions in Legume-Rhizobia Symbiosis and Root Architecture Control
Abstract
Nitrogen is an essential nutrient required for plant growth and the first nutrient which becomes limiting in agricultural fields. Symbiotic nitrogen fixation is an agronomically important biological process that results in natural input of nitrogen into agricultural soils. Legumes and soil bacteria called rhizobia that convert atmospheric nitrogen into readily available ammonia enter a partnership that leads to formation of specialized organs called nodules on legume roots. Small signaling peptides (SSPs) or peptide hormones are emerging regulators of root nodule formation and are fragments of larger polypeptides that range from five to 65 amino acids. SSPs are classified into subgroups such as post translationally modified peptides such as the GOLVEN/ROOT GROWTH FACTOR peptide family, Cysteine rich and Glycine Rich Peptides (GRPs). Although there are several studies investigating the first two subgroups, not much is known about the Glycine rich peptides. In the model legume Medicago truncatula there are 57 genes which encode glycine rich proteins, of which three have been implicated in rhizobial infection previously. Glycine rich peptides in nodules range between 60-250 amino acids and are characterized by an N-terminal secretion signal followed by stretches of glycine residues. The research is focused on the characterization of glycine rich proteins in root nodule development of M. truncatula, as they are induced during nodule development. Of the 57-glycine rich protein (NodGRP) genes, MtNodGRP31 is induced during late stages of nodule development upon inoculation with the rhizobia Sinorhizobium meliloti; these include 10 days post inoculation (dpi), 14dpi and 28dpi.
Subject Area
Plant sciences|Microbiology|Soil sciences|Agronomy
Recommended Citation
Manoj Kumar Reddy Allam,
"Investigating NodGRPs, GOLVEN10 Functions in Legume-Rhizobia Symbiosis and Root Architecture Control"
(2023).
ETD Collection for Tennessee State University.
Paper AAI30571551.
https://digitalscholarship.tnstate.edu/dissertations/AAI30571551