Development of an Electrochemical Sensor for the Detection of Glutathione (GSH)

Alfred P. Gbomina, Tennessee State University


Copper nanoparticles were successfully electro-deposited on graphene oxide nanosheets modified glassy carbon electrodes. The GCE/GO/CuNPs electrode was then coated with chitosan to obtain the GCE/GO/CuNPs/CS sensor. X-ray photoelectron spectroscopy (XPS) was used for surface characterization of the synthesized nanomaterials. The electro-catalytic activity of the fabricated sensor towards the oxidation of glutathione was monitored using cyclic voltammetry techniques. The fabricated sensor showed an optimum anodic current peak at a low-over potential voltage (+0.25 V vs Ag/AgCl) at pH = 5.0 in acetate buffer solution. Chronoamperometric analysis was performed at +0.25 V vs Ag/AgCl constant potential to investigate/monitor the current response of the fabricated sensor to various concentrations of glutathione. The I-t curve shows the robustness of the fabricated sensor. The calibration curve, obtained from the I-t result, was linear over a wide concentration range (0 to 2.80 mM) with high sensitivity (5.721 A/mM). A low LOD (20 M) was obtained based on 3 times the standard deviation of the blank divided by the slope of the linear curve. Overall, the results showed that CuNPs and GO triggered an outstanding synergistic effect with enhanced electrocatalytic activity towards the oxidation of glutathione. We have shown that electrochemical sensor materials can easily be fabricated for highly sensitive glutathione detection using the electro-deposition approach.

Subject Area

Biochemistry|Physical chemistry|Analytical chemistry

Recommended Citation

Alfred P. Gbomina, "Development of an Electrochemical Sensor for the Detection of Glutathione (GSH)" (2022). ETD Collection for Tennessee State University. Paper AAI29062918.