Anticancer Activity of Senna Leaf and Fenugreek Seed Against Two Cell Lines

Yousef Almutairi, Tennessee State University


Cancer is one of the leading causes of death worldwide. The most common cases of cancer are breast cancer, prostate cancer, leukemia, thyroid cancer, bladder cancer, and liver cancer. Cancer treatment represents a major challenge for governments and the health and pharmaceutical fields, due to the high cost in addition to the presence of side effects. Plants are a rich source of secondary metabolites, which contain therapeutic properties for many diseases, in addition to cancers. The sole purpose of the study was to determine the advantages of using natural cancer treatments such as the use of Senna Leaves and Fenugreek Seeds. Experimental method: different concentrations of extracts of Senna Leaves and Fenugreek Seeds were exposed to cancer cell lines taken from lung and prostate. Cell viability, Caspase-3 antagonist to detect apoptosis, and peroxidation of lipid assays as antioxidant activity were measured. It was found that the cell viability decreased in general with increasing concentration of extracts in PC3 and A549 cancer cells. MDA level activities were reduced demonstrating reduction of lipid peroxidation in the cancer cell. The study also showed an increase in the production of Caspase-3 enzyme in PC3 and A549 when the concentration of the extracts increased. The results showed that Senna Leaf extract and Fenugreek Seed had high efficacy against cancerous cells, which could be an effective treatment or prevention of cancers caused by PC-3 and A549 cell strains.

Subject Area

Molecular biology

Recommended Citation

Yousef Almutairi, "Anticancer Activity of Senna Leaf and Fenugreek Seed Against Two Cell Lines" (2022). ETD Collection for Tennessee State University. Paper AAI29060058.