Comparative analysis and characterization of proteins associated with fat accretion in broiler chickens and guinea fowl
One of the major goals in breeding of poultry is to improve growth performance and efficiency of feed utilization. However selection for rapid growth rate has resulted in excess fat deposition in broiler chickens, which is a liability to producers, processors, and consumers alike. Thus understanding the mechanisms surrounding excessive fat deposition in broilers is of great interest to the poultry and consumers. The adipose tissue proteome of broiler chickens and guinea fowl was evaluated using two-dimensional differential gel electrophoresis (2D-DIGE) followed by in-gel digestion and Matrix Assisted Laser Desorption/ionization Time-of-Flight (MALDI- TOF) mass spectrometry. The assays revealed proteins that were significantly up and down regulated in lean and fat broilers. These proteins include APOA1, vimentin, anexin, DSTN and fatty acid binding factor among others. While their functions are not very clear, these proteins may be involved directly or indirectly in fat metabolism and transportation. The objective of this study was to characterize adipose APOA1 and DSTN in broiler chickens and guinea fowl. The presence of the proteins was confirmed using western blot. Q-PCR assays were carried out on the adipose tissue of selected lean and fat birds for comparison of the expression level of the target proteins in both chickens and guinea fowl. APOA1 was found to be down regulated in fat chickens compared to lean chickens. In guinea fowl APOA1 was highly expressed in adipose tissue of fat birds compared to the lean birds. DSTN was found to be highly expressed in adipose tissue of fat birds compared to the lean birds in both chicken and guinea fowl. Other tissues were assayed to determine whether the expression of the proteins was localized or diffuse. Expression of APOA1 and DSTN was confirmed in the liver, intestines and pancreas of chicken. Here we report, for the first time, the expression of APOA1 and DSTN in the adipose, liver, intestines and pancreas of guinea fowl. Sequencing of the guinea fowl DSTN was performed using the amino acid sequence data. Here we report, for the first time, a partial 11 amino acid DSTN sequence YALYDASFETK. Multiple sequence alignment of the partial guinea fowl DSTN sequence revealed 100% homology with mammalian, avian and reptilian DSTN sequences (UniProt database).
Boniface Muthuri Kimathi,
"Comparative analysis and characterization of proteins associated with fat accretion in broiler chickens and guinea fowl"
ETD Collection for Tennessee State University.