Cytotoxic Extracts of Ethnomedicinal Plants: Podophyllum peltatum (Mayapple) and Echinacea angustifolia
Natural compounds are known historically as cures for many ailments in rural America and other developing countries. Our lab is testing plants, Echinacea angustifolia and Podophyllum peltatum are noted for their unique medicinal purposes and anticancer potential. The objectives of this study were to identify bioactive plants based on their ethnomedicinal usage, screen with cytotoxic assays to identify plants with potential anti-cancer properties, and compare viability of cell lines with both plants. I hypothesized that Podophyllum peltatum would show the best anticancer properties. Crude methanol extracts were tested against human metastatic cell lines representing breast (MCF-7), (BT-549) and colon (SW620) cells. Alamar Blue™ analysis was used to evaluate the cytoxicity of the extracts. Analysis of the crude extracts of Echinacea angustifolia on MCF-7, BT-549, and SW620, most of them exhibit anticancer activity against the cell lines at the maximum concentration of 15µg /µL. Diluted concentrations have demonstrated good inhibitory effects with IC 50 values ranging between 60-50% inhibition. Analysis of the crude extracts of Podophyllum peltatum has shown on MCF-7, BT-549 and SW620 exceptional anticancer activity against cell lines at higher concentrations ranging from 15-0.9 µg /µL with IC50 values ranging from 95-75% inhibition. Concluding, that both plant extracts display anticancer properties.
Plant biology|Toxicology|Surgery|Plant sciences|Oncology
Olena T James,
"Cytotoxic Extracts of Ethnomedicinal Plants: Podophyllum peltatum (Mayapple) and Echinacea angustifolia"
ETD Collection for Tennessee State University.