Partial characterization of the plasmid, pNY05, and antibiotic sensitivity of Rhodococcus erytheus
The plasmid, pNY05, was isolated from Rhodococcus erytheus. The plasmid was determined to be 20 Kb in size and to be associated with tight-banding RNA. On agarose gels, the RNA formed two bands which were 1.5 Kb and 1.0 Kb in size. The organism was found to be resistant to erythromycin, neomycin, and ampicillin and non-resistant to polymyxin B. In addition, it had the ability to develop resistance to several other antibiotics. Cloning the plasmid into the vector, pUC19, proved to be unsuccessful due to the plasmid's high guanine-cytosine (G-C) content. It is highly probable that the organism's theoretical Z-DNA is a result of its high G-C content. Further study is needed to fully characterize the organism and its plasmid in order to understand its genetic capability.
Judy Christine Cooper,
"Partial characterization of the plasmid, pNY05, and antibiotic sensitivity of Rhodococcus erytheus"
ETD Collection for Tennessee State University.