Genomics Versuse Phenomics Classification of Bacillus thuringiensis Strains Isolated From Middle Tenessee
Classification of Bacillus thuringiensis strains was initially conducted using Biological analyses, which assessed the biochemical capability of 68 strains of BT isolated from Middle Tennessee. By this method of classification, the strains were separated into four major clusters. The purpose of the work reported here was to determine whether assessment of the 16S gene sequence of 68 strains will result in similar clustering of these strains. It was hypothesized that relationship of the strains at the genotype level will not be like that of the phenotype since these are two different criteria. Genomic DNA was isolated from the strains and used to amplify the 16S gene in PCR with two primer sets, 27F and 1389R, and 519F and 1492R. It was found that PCR products were produced using the two primer sets. However, the 519F and 1492R PCR products gave the best sequencing results. Thus, it was concluded that this primer set was appropriate for generating PCR products that could be used to assess the 16S gene sequence for each Bt strain. Further studies included sequencing the PCR products and aligning these sequences to assess the relationship of the strains to one another. The results were shown in a phylogenetic tree for the 68 Bt strains which clarified that the relationship of the strains at the genotype level are not like that of the phenotype. It was concluded that the environment that each strain came from had a significant impact on the relationship of the strains to one another.
"Genomics Versuse Phenomics Classification of Bacillus thuringiensis Strains Isolated From Middle Tenessee"
ETD Collection for Tennessee State University.