Dibutyltin Alters Production of Interleukin 1 and Interleukin 6 in Human Immune Cells
The wide use of Butyltins (BTs) for industrial and agricultural purposes has led to global contamination and their bioaccumulation in aquatic and terrestrial animals. Dibutyltin (DBT) is used to stabilize plastics and as a deworming agent in some poultry. It is found in human blood (levels as high as 0.3 µM). Interleukin (IL) 1 beta (IL-1β) and IL-6 are important pro-inflammatory cytokines produced by lymphocytes, monocytes, and other cells. Both regulate cell growth, tissue repair, and immune functions. Increased levels of IL-1β and IL-6 have been associated with a number of diseases including rheumatoid arthritis and certain cancers. Recent studies in our lab have shown that DBT alters the secretion of IL-1β and IL6. DBT caused decreases in IL-1β and IL-6 secretion at higher concentrations while causing increases at lower concentrations. The aim of the current study is to determine whether alterations in IL-1β and IL-6 secretion are accompanied by changes in their production. Preparations of monocytes-depleted peripheral blood mononuclear cells were exposed to DBT at concentrations of 5, 2.5, 1, 0.5, 0.25, 0.1, and 0.05 µM for 1, 6 and 24 hours and the production (combination of Secreted and intracellular levels) of both IL-1β and IL-6 were measured. Results indicated that production of both cytokines remained relatively unchanged after 1 h and 6 h exposures to DBT. However, 24 h exposures to DBT generally decreased production of both IL-1β and IL-6 at the highest concentrations but increased production at lower concentrations. These data indicate that DBT-induced changes in the secretion of both of these cytokines are associated with an accompanying change in their intracellular production.
Linda B Sushak,
"Dibutyltin Alters Production of Interleukin 1 and Interleukin 6 in Human Immune Cells"
ETD Collection for Tennessee State University.